Identification of the gene involved in the anti-proliferative action of estrogen

2014 Fiscal Year Final Research Report

• Project/Area Number: 24590297
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Environmental physiology (including Physical medicine and Nutritional physiology)
• Research Institution: University of Yamanashi
• Principal Investigator: ARITA Jun 山梨大学, 総合研究部, 教授 (80128587)
• Co-Investigator(Renkei-kenkyūsha): MITSUI Tetsuo 山梨大学, 大学院総合研究部, 助教 (20402084) ; ISHIDA Maho 山梨大学, 大学院総合研究部, 助教 (80362086)
• Research Collaborator: WANG Linghong ; IZAWA Michi
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: エストロジェン / プロラクチン産生細胞 / 下垂体前葉 / Rasd1 / 細胞増殖

Outline of Final Research Achievements

The female ovarian steroid hormone estrogens have an anti-proliferative action as well as a well-known proliferative action on estrogen-responsive cells. We investigated the involvement of Rasd1, a tumor suppressor gene, in the regulation of the anti-proliferative action of estrogens in the present studies.
The expression of Rasd1 mRNA was stimulated at very early times after estradiol treatment of anterior pituitary cells in culture. The change in Rasd1 mRNA expression was accompanied by an increase in the promoter activity of the Rasd1 gene. Overexpression of Rasd1 inhibited proliferation of pituitary lactotrophs in culture while inhibition of estrogen-induced Rasd1 expression blocked the anti-proliferative action of estrogen.

Free Research Field

神経内分泌学