Regulation of NADPH oxidase gene: the role and meaning as a therapeutic target in septic encephalopathy.

2014 Fiscal Year Final Research Report

• Project/Area Number: 24590315
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General pharmacology
• Research Institution: Tokoha University (2014); University of Toyama (2012-2013)
• Principal Investigator: YOKOO Hiroki 常葉大学, 健康プロデュース学部, 教授 (30332894)
• Co-Investigator(Kenkyū-buntansha): HATTORI Yuichi 富山大学, 大学院医学薬学研究部(医学), 教授 (50156361) ; TAKANO Yasuo 東京工科大学, 医療保健学部, 教授 (60142022)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 炎症 / 酸化ストレス / 敗血症

Outline of Final Research Achievements

We focused on NADPH oxidase (Nox) activity and studied about sepsis encephalopathy. Proinflammatory cytokines and brain vessel permeability were significantly up-regulated in septic mice brain. And, Nox subunits, p47phox and p67phox, oxidative stress marker 8-OHdG, and iNOS expression were up-regulated in septic brains. These indicate that superoxide, produced by Nox, reacts with NO to form peroxynitrite, that might provoke a failed blood brain barrier. Light and electron microscopic examination showed that serious neuronal degeneration was occurred in septic mice brain. However, these histopathological changes were mitigated by treatment with the free radical scavenger edaravone. Thus, it was suggested that Nox gene might be therapeutic target for treating septic encephalopathy.

Free Research Field

基礎医学・薬理学一般