Study on the functions of PDZRN3, a novel regulator of adipogenesis, for the development of therapy for diabetes

2014 Fiscal Year Final Research Report

• Project/Area Number: 24590323
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General pharmacology
• Research Institution: Yamaguchi University
• Principal Investigator: HONDA Takeshi 山口大学, 医学(系)研究科(研究院), 講師 (30457311)
• Co-Investigator(Kenkyū-buntansha): INUI Makoto 山口大学, 大学院医学系研究科, 教授 (70223237)
• Co-Investigator(Renkei-kenkyūsha): KURAMASU Atsuo 山口大学, 大学院医学系研究科, 准教授 (90302091)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 脂肪細胞分化 / PDZRN3 / STAT5 / 3T3L1

Outline of Final Research Achievements

PDZRN3 is a novel regulator of mesenchymal progenitor cell differentiation. We previously demonstrated that PDZRN3 is essential for the differentiation of myoblasts into myotubes, whereas it suppresses the differentiation of osteoblasts. In this study, we clarified that PDZRN3 plays a pivotal role in adipocyte differentiation as a negative regulator. In 3T3-L1 cells, PDZRN3 suppressed the expression of STAT5b in a subtype-specific manner. STAT5b is a critical transcription factor for adipocyte differentiation. Furthermore, we extracted the proteins co-precipitated with PDZRN3 from the mouse embryonic tissues, and identified several candidates of a PDZRN3-interacting protein by mass spectrometry-based shotgun proteomic analysis.

Free Research Field

薬理学