Preparation of functional cell aggregates for transplantation

2014 Fiscal Year Final Research Report

• Project/Area Number: 24590365
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General medical chemistry
• Research Institution: Kwansei Gakuin University
• Principal Investigator: HIRAI Yohei 関西学院大学, 理工学部, 教授 (00397572)
• Research Collaborator: MAEKUBO Kenji ; AONO Yuichi ; MIYAZAKI Takafumi ; OJIMA Mariko ; HAGIWARA Natsumi ; YAMADA Muneharu ; MINA Bissell ; DEREK Radisky
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 細胞・組織 / 再生医学 / 生体機能利用 / 発生・分化

Outline of Final Research Achievements

This study aimed at the establishment of effective methods to prepare the functional cell aggregates by 1) generating cells (donor cells) that provide appropriate microenvironment for the functional differentiation of stem cells (accepter cells), 2) co-culture with donor and acceptor cells in three dimensional cell aggregates, and 3) effective removal of the donor cells. The processes of step 1 and 2 have been successful, however, the selective elimination of the donor cells (step 3) appeared to be difficult. Thus, an alternative approach was carried out, that is, the preparation of acceptor cells in which genes of proteins from the donor cells responsible for differentiation-induction were expressed under the strict control with tetracycline. By this approach, cell aggregates composed of functional cells, including cardiomyocytes, keratinocytes, chondrocytes and mammary epithelial cells, were prepared.

Free Research Field

細胞生物学、再生医学