The role of Leukotriene B4 receptor type 2 in skin barrier function.

2014 Fiscal Year Final Research Report

• Project/Area Number: 24590386
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Pathological medical chemistry
• Research Institution: Juntendo University (2014); Kyushu University (2012-2013)
• Principal Investigator: SAEKI KAZUKO 順天堂大学, 医学部, 准教授 (00553273)
• Research Collaborator: LIU Min ; ISHII Yumiko
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 脂質 / GPCR / 皮膚

Outline of Final Research Achievements

Leukotriene B4 receptor type 2 (BLT2) is a G protein-coupled receptor (GPCR) highly expressed in epidermal keratinocytes. 12-HHT is a high affinity ligand for BLT2 and produced during blood coagulation in a COX/TxA2S-dependent manner. In this study, we found that 12-HHT/BLT2 axis promotes skin wound healing by accelerating keratinocyte migration. It has been known that aspirin, a COX inhibitor, delays skin wound healing, but the precise mechanisms were wholly unknown. We showed that the impairment of 12-HHT production by aspirin treatment delays wound healing. We also showed that topical application of synthetic BLT2 agonist accelerates skin wound healing in wild-type C57BL/6J mice and diabetic db/db mice. These results suggest the possible application of BLT2 agonist as a therapeutic agent to accelerate wound healing, particularly for intractable wounds, such as diabetic ulcers.

Free Research Field

生化学、免疫学