General transcription factor TAF1 is involved in transcriptional impairment and neurodegeneration

2014 Fiscal Year Final Research Report

• Project/Area Number: 24590400
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Human genetics
• Research Institution: Tohoku University (2013-2014); Yamagata University (2012)
• Principal Investigator: MAKINO Satoshi 東北大学, 東北メディカル・メガバンク機構, 研究支援者 (30423403)
• Co-Investigator(Kenkyū-buntansha): TOOYAMA Ikuo 滋賀医科大学, 分子神経科学研究センター, 教授 (20207533)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 転写因子 / 神経変性 / 転写障害

Outline of Final Research Achievements

We performed cell-cycle analysis in TAF1 or neuron-speciphic TAF1 (N-TAF1) transfected temperature-sensitive mutant hamster cell line, exhibits cell cycle arrest and apoptosis at the restrictive temperature. The aim is the comparison of the functional difference between TAF1 and N-TAF1 according to the view of possibilities that transcriptional impairment of subunit of general transcription factor causes neurodegeneration. Analysis of cell cycle by flow cytometry in TAF1 or neuron-speciphic TAF1 (N-TAF1) transfected mutant hamster cell line showed that the point mutation affects cell cycle in the cell line is less relevant to TAF1's neuron-specific functions.

Free Research Field

分子遺伝学