2014 Fiscal Year Final Research Report
Expression cloning of the gene functioning in transformation of Caco-2 cells to M cells
| Project/Area Number |
24590410
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Human pathology
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| Research Institution | University of Fukui (2013-2014)
Shinshu University (2012) |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KOJIMA Katsuhiko 信州大学, 医学部, 助教 (80345743)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | M細胞 / 発現クローニング |
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| Outline of Final Research Achievements |
Our research goal was to clone the gene that transforms Caco-2 cells into M cells, which transcytose antigenic molecules in the intestinal lumen to subepithelial immune cells, by employing the Caco-2/Raji cells co-culture system. Furthermore, we aimed to establish monoclonal antibodies recognizing the gene products to clarify changes in the number and distribution of M cells in inflammatory bowel diseases. However, due to the difficulty to obtain the optimal condition for the induction of M cell phenotype, we changed the strategy. We tried to establish monoclonal antibodies against GP2 and Spi-B, both of which are found to be markers for mouse M cells. We have not yet obtained the hybridoma, and currently, we are preparing obtaining optimal condition for hybridoma screening.
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| Free Research Field |
糖鎖病理学
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