Pathological analysis of prognosis factor of follicular lymphoma

2014 Fiscal Year Final Research Report

• Project/Area Number: 24590430
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Human pathology
• Research Institution: Tokai University
• Principal Investigator: NAKAMURA Naoya 東海大学, 医学部, 教授 (50227922)
• Co-Investigator(Renkei-kenkyūsha): CARRERAS Joaquim 東海大学, 医学部, 講師 (90637191)
• Research Collaborator: KIKUTI Yara Yukie 東海大学, 医学部, 研究員
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 濾胞性リンパ腫 / 腫瘍関連ﾏｸﾛﾌｧｰｼ / cDNA microarray / Gene Set Enrichment解析 / 免疫組織化学

Outline of Final Research Achievements

Out of 108 genes identified by gene set enrichment analysis and functional association network analysis for Follicular lymphoma (FL), we focused 4 molecules of BTLA for T-cells, HVEM for dendritic cells, FOXP3 for regulatory T-cells and PD-1 for follicular helper T-cells. We also retrieved 4 molecules as tumor associated macrophages (TAM) of CD68 for pan-macrophage, CD16 for M1-like, and MiTF & CD163 for M2-like. Immunohistochemistry using formalin-fixed paraffin-embedded sections of low grade FL (LG-FL), high grade FL (HG-FL), diffuse large B-cell lymphoma transformed from FL (tDLBCL）was performed. Each of CD68+ cells and MiTF+ cells was increased in HG-FL other than LG-FL. PD-1+ cells was decreased in transformed DLBCL compared to LG-FL and HG-FL. Each of CD68+ cells and CD163+ cells was increased. Therefore, microenvironment including macrophages, dendritic cells and T-cells have important role for progression of FL and transformation to DLBCL.

Free Research Field

人体病理学