Identification and analysis of the stem cells and its differentiation in sympathetic neural crest cells and neuroblastoma cells

2014 Fiscal Year Final Research Report

• Project/Area Number: 24590434
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Human pathology
• Research Institution: The Japanese Red Cross Toyota College of Nursing
• Principal Investigator: KUROKAWA Kei 日本赤十字豊田看護大学, 看護学部, 教授 (90399030)
• Co-Investigator(Kenkyū-buntansha): IWASHITA Toshihide 浜松医科大学, 医学部, 教授 (00283432) ; MURAKAMI Hideki 愛知医科大学, 医学部, 准教授 (90303619) ; SAGA Shinsuke 愛知医科大学, 医学部, 教授 (40144141)
• Co-Investigator(Renkei-kenkyūsha): TAKAHASHI Yoshiyuki 名古屋大学, 医学部, 准教授 (40432273)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 神経芽細胞腫 / 表面マーカー / 幹細胞

Outline of Final Research Achievements

In neuroblastoma cell lines, morphological transconversion between cells with neuronal process (N-type) and flat cells (S-type) are observed. Transconversion of cell surface markers correlating with morphological changes and differentiation of neuroblastoma cells were found. From several transconverting cell fractions, a cell population with a fixed combination of cell surface markers was derived. This population was morphologically N-type with rapid proliferation. It was suggested that this population forms a lower rank of differention than transconverting cell populations from the view point of cell surface markers. A definite hierarchy like developmental process of neural crest cell differentiation was not observed in neuroblastoma.

Free Research Field

実験病理学