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2014 Fiscal Year Final Research Report

Analysis of Regulatory Mechanism of RANK Gene Expression in Osteoclast Precursor Cells

Research Project

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Project/Area Number 24590461
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Experimental pathology
Research InstitutionEhime University

Principal Investigator

KITAZAWA RIKO  愛媛大学, 医学部附属病院, 准教授 (00273780)

Project Period (FY) 2012-04-01 – 2015-03-31
Keywords破骨細胞 / RANK / 遺伝子プロモータ / 転写因子
Outline of Final Research Achievements

Osteoclasts are multinucleated giant cells specialized in bone resorption, and are the major therapeutic target in diseases such as Osteoporosis and metastatic bone tumor. Receptor activator of NF-κB (RANK) is a member of the tumor necrosis factor receptor (TNFR) family expressed in osteoclast precursors, and RANK-RANK ligand (RANKL) signaling is a key system for differentiation, during differentiation of bone marrow mono-nucleated cells into osteoclast precursors. We had cloned a 6-kb fragment containing the 5’-flanking region of the RANK gene and have analyzed the binding elements of transcription factors. RANK transcription was positively regulated by c-fos/AP-1. We have identified splicing variant of mouse and human RANK gene (vRANK) that contains an intervening exon between exons 1 and 2 of full-length RANK (fRANK) mRNA. Since this novel exon contains a stop codon, vRANK encodes short truncated amino acids. We have started to generate a vRANK-overexpressed transgenic mouse.

Free Research Field

病理学

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Published: 2016-06-03  

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