Analysis of molecular mechanism of oxidative stress regulation by RNA splicing

2015 Fiscal Year Final Research Report

• Project/Area Number: 24590464
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Experimental pathology
• Research Institution: Tokai University
• Principal Investigator: TAKEKOSHI Susumu 東海大学, 医学部, 教授 (70216878)
• Co-Investigator(Renkei-kenkyūsha): NAKAMURA Naoya 東海大学, 医学部, 教授 (50227922) ; TAKIZAWA Shunya 東海大学, 医学部, 教授 (70197234) ; HORIKOSHI Yosuke 東海大学, 医学部, 研究員 (60448678)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Keywords: 酸化ストレス / RNAスプライシング / シグナル伝達

Outline of Final Research Achievements

Diacylglycerol (DAG) is a physiological activator of protein kinase C (PKC). We reported that oxidized diacylglycerol (DAG-OOH) had a remarkably stronger PKC-activating action than un-oxidized DAG. DAG-OOH also induces neuronal cell injury through the over-activation of PKC delta molecule. On the other hand, PKC delta splicing variant (PKCSV) could prevent DAG-OOH-induced neuronal cell injury. In this study, the action of PKCSV to prevent oxidative stress- induced neuronal cell injury was analyzed and the expression of PKCSV under various oxidative stress arising was observed. In consequence, PKCSV over-expressing neuronal cells resistance to cell injury evoked by superoxide radical, hydrogen peroxide and DAG-OOH. Furthermore the expression of PKCSV significantly increased in oxidative stress models These result suggested that PKCSV has a protective action against oxidative stress-induce cell injury and the cell defense mechanism through the RNA splicing may exist.

Free Research Field

分子病理学