2014 Fiscal Year Final Research Report
Development of DNA demethylation technology in cancer cells and its application for discovering biomarkers for early diagnosis
| Project/Area Number |
24590471
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Experimental pathology
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | 腫瘍 / DNAメチル化 / DNA脱メチル化 |
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| Outline of Final Research Achievements |
We have developed a novel method that globally demethylates and reactivates hypermethylated genes by using a fusion gene comprising of methyl-CpG binding domain (MBD) and the catalytic domain of Ten-eleven translocation protein 1 (TET1-CD). This method is useful for identifying hypermethylation-mediated silenced genes in cancer cells. We have applied it to prostate and colorectal cancer cell lines and found that DNA demethylation suppressed the growth of cancer cells. These results suggest that DNA methylation confers the ability of cancer cells to facilitate the growth and DNA methylation of gene(s) responsible for cell growth may be useful as a biomarker.
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| Free Research Field |
実験病理学
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