2014 Fiscal Year Final Research Report
Significance of REV7 for germ cell maintenance and spermatogenesis
| Project/Area Number |
24590479
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Experimental pathology
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| Research Institution | Kitasato University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | REV7 / 精子形成 / DNA損傷 / コンディショナルノックアウトマウス / トランスジェニックマウス / 精巣 |
|---|
| Outline of Final Research Achievements |
To make clear roles of REV7 in spermatogenesis in the testis, we tried to generate Rev7 conditional knockout mouse, in which Rev7 could be deleted in the adult period. Because we could not generate the mouse in our first plan, we purchased commercially-available gene targeted mouse and are now generating the Rev7 conditional knockout mouse. We performed another project to identify REV7-interacting proteins in the testis using Rev7 transgenic mouse testes. Although several candidate bands were detected in the immunoprecipitation analysis, we could not analyze them by mass spectrometry because of less amount of the proteins. We will continue the project in the future.
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| Free Research Field |
実験病理学
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