Role of aPKC molecules in spermatogonial stem cell homing

2014 Fiscal Year Final Research Report

• Project/Area Number: 24590481
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Experimental pathology
• Research Institution: Shinshu University (2014); Kyoto University (2012-2013)
• Principal Investigator: TAKASHIMA Seiji 信州大学, 学術研究院繊維学系, 助教 (40396891)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 精子幹細胞 / ホーミング / 血液精巣関門

Outline of Final Research Achievements

Spermatogonial stem cells (SSCs) are the foundation of spermatogenesis and can colonize genetically infertile testes. Previous result demonstrated that Rac1 mediated CLDN3 expression is necessary for homing of SSCs. Although atypical PKC (aPKC) molecule, such as PKCl and PKCz are known to regulate expression of tight junction molecules including CLDN3, Role of aPKC in SSC homing remained unknown.
In the present study, we tried to unveil the role of aPKC molecules in SSC homing using aPKC conditional knockout mice. However, our SSC transplantation assay revealed that aPKC molecules were dispensable for SSC homing.

Free Research Field

生殖生物学　幹細胞生物学　再生医学