Involvement of lincRNA in carcinogenesis

2014 Fiscal Year Final Research Report

• Project/Area Number: 24590489
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Experimental pathology
• Research Institution: Iwate Medical University
• Principal Investigator: SHIBAZAKI Masahiko 岩手医科大学, 医学部, 助教 (20445109)
• Co-Investigator(Kenkyū-buntansha): MAESAWA Chihaya 岩手医科大学, 大学院医学研究科, 教授 (10326647) ; AKASAKA Toshihide 岩手医科大学, 大学院医学研究科, 教授 (30137525) ; SUGIYAMA Toru 岩手医科大学, 大学院医学研究科, 教授 (40162903) ; KASHIWABA Masahiro 岩手医科大学, 大学院医学研究科, 講師 (80326660)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 非翻訳RNA / 腫瘍化

Outline of Final Research Achievements

In epithelial ovarian cancer cell lines and primary tumors, we investigated that miR-10b and/or HOTAIR can regulate the HOXD10 expression, and that it permit gain of pro-metastatic gene products. Our results suggested that downregulation of HOXD10 expression by miR-10b overexpression might induce increase of pro-metastatic gene products, such as MMP14 and RHOC, and contribute to the acquisition of metastatic phenotypes in epithelial ovarian cancer cells. Moreover, We identified a novel single-nucleotide deletion in codon 507 of exon 4 of the KEAP1 gene that resulted in a frameshift mutation in malignant melanoma. KEAP1-FSM cells exhibited significant resistance to hydrogen peroxide and anti-cancer agents such as cisplatin and dacarbazine, which are mostly used for melanoma chemotherapy. These data suggest that NRF2 may have potential value as a therapeutic target in malignant melanoma in order to improve the rate of clinical response to anti-cancer agents.

Free Research Field

分子生物学