Development of novel biomarker for diagnosis, prognosis and therapy of opisthorchiasis-associated cholangiocarcinoma

2014 Fiscal Year Final Research Report

• Project/Area Number: 24590504
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Parasitology (including Sanitary zoology)
• Research Institution: Gifu University
• Principal Investigator: WU ZHILIANG 岐阜大学, 医学(系)研究科(研究院), 助教 (90313874)
• Co-Investigator(Kenkyū-buntansha): NAGANO Isao 岐阜大学, 大学院医学系研究科, 准教授 (40283296)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: タイ肝吸虫 / 胆管癌 / 腫瘍マーカー / MFGE8 / S100P / 癌診断 / 抗癌剤標的

Outline of Final Research Achievements

The present study identified novel cancer markers for opisthorchiasis-associated cholangiocarcinoma (CCA). After further study on the candidates listed up based on our previous cDNA microarray analysis, we focused on the two markers, MFGE8 and S100P. The early elevation of expression in Opisthorchis viverrini infection-induced cholangiocarcinoma (CCA) animal model, intensive expression in the precancerous lesion of hyperplasia and dysplasia in bile ducts and tumor cell, and high levels in serum and bile fluid indicated that both can be novel markers for CCA diagnosis. The high expression could be an independent prognostic factor. The shRNA-mediated knockdown of S100P expression inhibited the proliferation of CCA, arrested cell cycle, promoted apoptosis and increased the sensitivity to chemotherapeutic agents. This study indicates that MFGE8 and S100P are promising novel biomarker for diagnosis, prognosis and therapy of CCA.

Free Research Field

寄生虫学