2014 Fiscal Year Final Research Report
Ultrastructural analysis of Maurer's clefts of Plasmodium falciparum infected red blood cell
| Project/Area Number |
24590508
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Parasitology (including Sanitary zoology)
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| Research Institution | Nagasaki University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
YAHATA Kazuhide 長崎大学, 熱帯医学研究所, 助教 (40467965)
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| Co-Investigator(Renkei-kenkyūsha) |
KANEKO Osamu 長崎大学, 熱帯医学研究所, 教授 (50325370)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | マラリア / 感染赤血球 / 免疫染色 / 電子顕微鏡 |
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| Outline of Final Research Achievements |
To clarify the formation process and ultrastructure of Maurer’s clefts in the Plasmodium falciparum infected red blood cell, we generated transgenic P. falciparum lines expressing Pfmc2TM fused with GFP and several epitope tags such as Flag or Myc. We found that the protein localized to the Maurer’s clefts. However, we could not use the transgenic lines for live imaging by confocal microscopy, since the GFP signal was very low intensity. Next, we used SBF-SEM to image the 3D structure of multiple entire P. falciparum infected red blood cells at each stages. The 3D organization showed that Maurer’s clefts are not connected to the parasitophorous vacuole, red blood cell membrane, or tubovesiclar network. They are not continuous membrane networks but independent membranous structures in the red blood cell.
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| Free Research Field |
細胞生物学
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