Analysis of unique lysosomal soluble protein transporting receptor family proteins in enteric protozoan parasite Entamoeba histolytica

2014 Fiscal Year Final Research Report

• Project/Area Number: 24590513
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Parasitology (including Sanitary zoology)
• Research Institution: National Institute of Infectious Diseases
• Principal Investigator: TSUKUI Kumiko 国立感染症研究所, 寄生動物部, 主任研究官 (00420092)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: lysosome / receptor / traffic / Entamoeba histolytica

Outline of Final Research Achievements

Lysosomal soluble proteins are targeted to endosomes and lysosomes by specific receptors. The enteric protozoan parasite Entamoeba histolytica has a novel class of lysosomal targeting receptors, named the cysteine protease binding protein family (CPBF). Among 11 CPBFs, CPBF1 is a solo receptor for cysteine proteases and other 6 CPBFs recognize glycolytic enzymes, i.e. amylases, β-hexosaminidases, and lysozymes. It was shown by bioinformatics analysis and phylogenetic reconstruction that each CPBF contains six prepeptidase carboxyl-terminal domains, and the domain configuration is evolutionarily conserved among CPBFs. Taken together, CPBFs with unique and conserved domain organisation have a remarkable ligand heterogeneity toward cysteine protease and carbohydrate degradation enzymes. These findings add new insights into the unique molecular event in enteric protozoan pathogen E. histolytica.

Free Research Field

分子寄生虫学