Positive and negative regulation of cytokine signals in basophils

2014 Fiscal Year Final Research Report

• Project/Area Number: 24590578
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Immunology
• Research Institution: Shinshu University
• Principal Investigator: TAKI Shinsuke 信州大学, 学術研究院医学系, 教授 (50262027)
• Co-Investigator(Renkei-kenkyūsha): HIDA Shigeaki 信州大学, 学術研究院医学系, 准教授 (10345762) ; SANJO Hideki 信州大学, 学術研究院医学系, 助教 (50391967)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: サイトカイン / 好塩基球 / サイトカインレセプター / 細胞内シグナル伝達

Outline of Final Research Achievements

Structure-function relationship in the beta-c subunit of the IL-3 receptor for the intracellular signals for IL-4 production was investigated. In the past, technical limitations made studies of the beta-c cytoplasmic structure confined to those for survival/growth signals. We here developed a new experimental system that enabled us to examine the roles of beta-c substructures for IL-4 production as well as for survival/growth in primary murine basophils. As previously shown, beta-c lacking the entire cytoplasmic region or the so-called box1 region, to which Jak2 kinase bound, failed to transduce IL-3 signals for survival/growth and IL-4 production. In contrast, beta-c mutants lacking the more distal box2 region or with Phe-to-Tyr substitution at position 573 could support basophil survival/growth normally but were inefficient in triggering IL-4 production. This study is the first to identify unique beta-c subregions important for the IL-3 signals for effector functions.

Free Research Field

免疫学