Clinical application of the rapid IT system for identifying pathogenic bacteria using the eukaryote-made Taq DNA polymerase

2014 Fiscal Year Final Research Report

• Project/Area Number: 24590682
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Laboratory medicine
• Research Institution: University of Toyama
• Principal Investigator: NIIMI HIDEKI 富山大学, 大学病院, 助教 (50401865)
• Co-Investigator(Kenkyū-buntansha): KITAJIMA Isao 富山大学, 大学院医学薬学研究部(医学), 教授 (50214797) ; NOTE Ryougou 富山大学, 大学病院, 臨床検査技師長 (60377364)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 敗血症 / 感染症 / 遺伝子検査 / PCR / 迅速検査

Outline of Final Research Achievements

We developed the novel IT system (Tm mapping method) for rapidly identifying the dominant bacteria in a clinical sample. Employing only seven primer sets, more than 100 bacterial species can be identified. Moreover, this method can be used to rapidly diagnose the absence of bacteria in clinical samples.
We tested the Tm mapping method using 200 whole blood samples obtained from patients with suspected sepsis, 85% (171/200) of which matched the culture results based on the detection level. A total of 130 samples were negative according to the Tm mapping method, 98% (128/130) of which were also negative based on the culture method. Meanwhile, 70 samples were positive according to the Tm mapping method, and of the 59 suitable for identification, 100% (59/59) exhibited a “match”with the culture or sequencing results. These findings were obtained within three hours of whole blood collection. The Tm mapping method is useful for identifying infectious diseases requiring prompt treatment.

Free Research Field

感染症検査方法の開発