Development of a redox biological reaction-based diagnostic method at bedside for oxidative stress

2014 Fiscal Year Final Research Report

• Project/Area Number: 24590686
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Laboratory medicine
• Research Institution: University of Fukui
• Principal Investigator: KASUNO Kenji 福井大学, 医学部, 准教授 (60455243)
• Co-Investigator(Kenkyū-buntansha): IWANO Masayuki 福井大学, 医学部, 教授 (20275324) ; KIMURA Hideki 福井大学, 医学部附属病院, 准教授 (20283137) ; TKAHASHI Naoki 福井大学, 医学部附属病院, 助教 (30377460) ; NAKAMURA Hajime 公益財団法人田附興風会, 医学研究所第8研究部, 研究員 (70303914)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 酸化ストレス / 急性腎障害 / 尿中チオレドキシン

Outline of Final Research Achievements

We found that urinary thioredoxin (TRX) levels were markedly higher in patients with acute kidney injury (AKI) than in those with chronic kidney disease or in healthy subjects. In a steady state, TRX in blood is filtered and reabsorbed by renal tubular cells. During kidney injury, tubular cells excrete TRX in an oxidative form in response to oxidative stress. With using this unique phenomenon, we developed a redox biological reaction-based diagnostic method at bedside for oxidative stress. This method employed chemiluminescent immuno assay, and completed within 15 minutes. For the differentiation between AKI and other renal diseases, the area under the curve for urinary TRX was 0.94 (95% confidence interval, 0.90-0.98) in a receiver operating characteristic curve analysis, and the sensitivity and specificity were 0.88 and 0.88, respectively, at the optimal cutoff value of 43.0 ug/g creatinine. This diagnostic ability is comparable to established AKI markers NGAL, KIM-1, and L-FABP.

Free Research Field

酸化ストレス