2014 Fiscal Year Final Research Report
Molecular analysis of pathogenesis and the unique machinery of cell division in Helicobacter pylori
| Project/Area Number |
24590697
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | Kochi University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KUMON Yoshio 高知大学, 教育研究部医療学系, 准教授 (40215033)
SUGIURA Tetsuro 高知大学, 教育研究部医療学系, 教授 (50171145)
MORIMOTO Norihito 高知大学, 医学部附属病院, その他 (60398055)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | ヘリコバクター・ピロリ / 細胞分裂制御システム / 形態形成 / minCDE / ftsZ / cdrA / coccoid |
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| Outline of Final Research Achievements |
Bacterial cells precisely divide, coordinated by many molecular components including Min proteins and FtsZ. However, the role of Min proteins in H. pylori is little known. We investigated the function of Min proteins with a wild-type HPK5 and HPK5-derivative mutants constructed. All disruptants were filamentous with few minicells. These genes maintained the cell morphology. The lowest coccoid form appeared in minE-disruptant, indicating that MinE contributes to the coccoid conversion at the stationary phase. FtsZ was dispersed throughout a cell in only minD-disruptant by immunofluorescence microscopy, revealing that MinD is involved in conduct of FtsZ localization. These showed the intrinsic characteristics of H. pylori Min proteins and provided new insights to understand the cell division of H. pylori.
Furthermore, we first discovered new spherical phages (KHP30 and KHP40) from 2 clinical isolates.
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| Free Research Field |
臨床検査医学（微生物・感染症学）
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