2016 Fiscal Year Final Research Report
Analysis of phenotype and genotype of antibiotic resistant bacterial isolates whose resistance mechanism was not unraveled by routine laboratory examination
| Project/Area Number |
24590708
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | Showa University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2017-03-31
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| Keywords | metallo beta lactamase / carbapenem / Enterobacter cloacae / Klebsiella pneumoniae / Pseudomonas aeruginosa / Acinetobacter baumannii / multi-drug resistant / Enterobacteriaceae |
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| Outline of Final Research Achievements |
Molecular epidemiological analysis was performed for antimicrobial resistant bacteria isolated in our hospital. Many carbapenem resistance gene positive enterobacteriaceae were isolated in the same period and the same ward. The result of the antimicrobial susceptibility testing of those isolate showed both carbapenem susceptible and resistant phenotypes. Those isolates showed the identical genotype on Pulsed-field gel electrophoresis, indicating the nosocomial spread of the strain.
Further, colonization of Metallo beta lactamase gene positive Multi drug resistant Pseudomonas aeruginosa strains with same genotype in our hospital was revealed.
The isolates of carbapenem resistant Acinetobacter baumannii were analyzed genetically. Multi Locus Sequence Typing revealed that the strain belonged Clonal Complex 92 as the global clone, and analysis of resistant gene identified the OXA23 beta lactamase gene.
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| Free Research Field |
臨床検査医学 臨床微生物学
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