Development of homogeneous assay for HDL cholesterol subfractions and assessment for clinical application

2014 Fiscal Year Final Research Report

• Project/Area Number: 24590717
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Laboratory medicine
• Research Institution: Kumamoto Health Science University
• Principal Investigator: Sugiuchi Hiroyuki 熊本保健科学大学, 保健科学部, 教授 (70435163)
• Co-Investigator(Kenkyū-buntansha): 松嶋 和美 熊本保健科学大学, 保健科学部, 講師 (00369125) ; 安東 由喜雄 熊本大学, その他の研究科, 教授 (20253742) ; 安楽 健作 熊本保健科学大学, 保健科学部, 講師 (80389543)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: コレステロール / HDL亜分画コレステロール / ホモジニアス法 / HDL2-コレステロール / HDL3-コレステロール

Outline of Final Research Achievements

In this study, we found that addition of the cationic surfactant and a polyethylene glycol modified enzyme to the cholesterol assay system selectively solubilizes HDL3-C. We then applied this knowledge to the development of a homogeneous HDL3-C assay that is simple and requires no separation processing. The repeatability of this method was found to be 2.0% or less by CV% for HDL3-C in the range of 10 to 30 mg/dl, and its coefficient of correlation with the subfraction by ultracentrifugation was r=0.841 with the regression equation y=0.884x＋4.807 for n=20. The method can be embodied in an automatic analyzer for simple, fast measurement of HDL3-C using a minute amount of sample, and is expected to substantially contribute to the treatment and prevention of arteriosclerotic disease.

Free Research Field

医歯薬学