Analysis of genetic mechanism for reduced nociceptive response by genome-wide gene expression assay

2014 Fiscal Year Final Research Report

• Project/Area Number: 24590741
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Pain science
• Research Institution: Tokyo Metropolitan Institute of Medical Science
• Principal Investigator: KASAI Shinya 公益財団法人東京都医学総合研究所, 精神行動医学研究分野, 主席研究員 (20399471)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 疼痛感受性 / 鎮痛薬感受性 / 全ゲノム遺伝子発現 / モデルマウス

Outline of Final Research Achievements

CXBH mice exhibited slightly higher morphine-induced antinociception compared with C57BL/6J and BALB/cBy mice in the hot-plate test but not tail-flick test. CXBH mice exhibited a marked reduction of nociceptive sensitivity, regardless of the type of nociceptive stimulus, with the exception of tail stimulation. Changes in gene expression that corresponded to reduced nociceptive sensitivity in the brains of CXBH mice were observed in 62 transcripts, including pain- and analgesia-related transcripts, in a whole-genome expression assay. The total mRNA expression of opioid receptors was higher in CXBH mice than in C57BL/6J and BALB/cBy mice. However, the expression levels of MOR-1 mRNA, a major transcript of the μ opioid receptor gene, were not different among the C57BL/6J, BALB/cBy, and CXBH strains. In conclusion, supraspinal nociceptive responses were reduced in the CXBH mouse strain, and the expression levels of transcripts were altered in the brain of this strain.

Free Research Field

神経精神薬理学