Development of bioinformatics tool to predict the hepatotoxicity of chemicals

2014 Fiscal Year Final Research Report

• Project/Area Number: 24590743
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Hygiene
• Research Institution: University of Toyama
• Principal Investigator: INADERA Hidekuni 富山大学, 大学院医学薬学研究部(医学), 教授 (10301144)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 化学物質 / 毒性学

Outline of Final Research Achievements

The development of systems for analyzing global expression patterns by molecular profiling techniques provides opportunities to establish the basis of toxicological responses. The general profile of gene expression provides insights into the molecular mechanisms by which chemicals might exert toxic effects. We developed a customized DNA microarray system and analyzed gene expression changes in response to representative chemicals. In vitro hepatotoxicity prediction systems using primary cultured hepatocytes have been used in this study. We found in vitro toxicity systems cannot fully reflect the in vivo expression profile and cellular function. We tried to establish the bioinformatics tool which can predict the in vivo response by using the transcriptome data of in vitro systems.

Free Research Field

公衆衛生学