Genotyping of insertion/deletion polymorphisms using locked nucleic acids for human identification

2014 Fiscal Year Final Research Report

• Project/Area Number: 24590847
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Legal medicine
• Research Institution: Asahikawa Medical College
• Principal Investigator: ASARI Masaru 旭川医科大学, 医学部, 准教授 (40360979)
• Co-Investigator(Kenkyū-buntansha): OMURA Tomohiro 京都大学, 医学(系)研究科(研究院), 助教 (00430935) ; OKA Kumiko 旭川医科大学, 医学部, 助教 (60571891)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 個人識別 / 挿入欠失多型 / 人工核酸LNA / ユニバーサルプライマー

Outline of Final Research Achievements

We analyzed the effects of locked nucleic acids (LNAs) for the efficiency of fluorescent labeling of PCR products. When the 24-base universal sequence with a higher Tm value was used, the introduction of a few LNAs significantly improved the efficiency, compared to the results from the 18-base universal sequence. We also selected 37 autosomal insertion/deletion (Indel) polymorphisms, and calculated the frequencies of alleles from 100 Japanese individuals. The matching probability was 2×10(-15), and the combination of selected Indels would be useful for individual discrimination. In multiplex Indel genotyping, fluorescent universal primers with LNAs produced higher signals than non-LNA fluorescent universal primers, and the increasing effects were identified especially in the low concentration (0.1 μM) of these primers.

Free Research Field

法医学