Development of analytical methods for the determination of drugs and poisons with molecularly imprinted polymers and its forensic application

2016 Fiscal Year Final Research Report

• Project/Area Number: 24590865
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Legal medicine
• Research Institution: Seirei Christopher University (2016); Showa University (2012-2015)
• Principal Investigator: KUMAZAWA Takeshi 聖隷クリストファー大学, 看護学部, 教授 (00186470)
• Project Period (FY): 2012-04-01 – 2017-03-31
• Keywords: 固相抽出 / 分子インプリントポリマー / 薬毒物分析 / 分離分析法

Outline of Final Research Achievements

In the present study, we examined the determination of drugs and poisons in human body fluids using molecularly imprinted solid-phase extraction (MISPE) as highly selective sample clean-up technique. After MISPE, the analytes extracted from whole blood, plasma, and urine were quantified using gas chromatography (GC), high performance liquid chromatography (HPLC) , GC-mass spectrometry (MS), HPLC-MS. The results showed that drugs and poisons can be efficiently extracted from crude biological samples by MISPE with relatively good reproducibility. The present analytical method will be a useful for the screening and quantitative determination of xenobiotics, in clinical and forensic analysis.

Free Research Field

医歯薬学