Study on the threshold for brain damage due to carbon monoxide poisoning

2014 Fiscal Year Final Research Report

• Project/Area Number: 24590866
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Legal medicine
• Research Institution: Tokyo Medical University
• Principal Investigator: HARA SHUICHI 東京医科大学, 医学部, 准教授 (70208651)
• Co-Investigator(Renkei-kenkyūsha): KURIIWA Fumi 東京医科大学, 医学部, 助手 (30468665)
• Research Collaborator: KOBAYASHI Masamune 東京医科大学, 医学部, 助教 ; MIZUKAMI Hajime 東京医科大学, 医学部, 准教授 ; MUKAI Toshiji 聖マリアンナ医科大学, 医学部, 教授 ; IKEMATSU Kazuya 長崎大学, 医学部, 教授
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 一酸化炭素中毒 / ヒドロキシルラジカル / NADPH oxidase / dual oxidase 2 / mRNA / ラット / 線条体

Outline of Final Research Achievements

We found that severe, but not non-severe, carbon monoxide (CO) poisoning enhanced mRNA expression of dual oxidase 2, which is a member of NADPH oxidase family involved in oxidative stress, in rat striatum. No such enhancement of mRNA expression was observed for other members of NADPH oxidase family. Inhibition of NADPH oxidase, but not xanthine oxidase, suppressed production of active oxygen species induced not only by severe CO poisoning, but also by enhancement of intracellular cAMP production due to forskolin. These findings suggest that NADPH oxidase may be under the control of cAMP signaling and contribute to brain damage due to severe CO poisoning. It is likely that dual oxidase 2 plays a role on brain damage. It is necessary to further examine the role of dual oxidase 2.

Free Research Field

中毒学