Study of the individual identity from the sample which used for the forensic detection by direct PCR method

2014 Fiscal Year Final Research Report

• Project/Area Number: 24590867
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Legal medicine
• Research Institution: Nihon University
• Principal Investigator: TIE Jian 日本大学, 医学部, 専任講師 (40277439)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 法科学試料 / 予備検査 / DNA多型 / 直接PCR増幅 / 個人識別

Outline of Final Research Achievements

A purpose of this study is to detect DNA polymorphism using the direct PCR method from a forensic sample or a sample which used for forensic medicine pretest. The normal forensic sample includes a bloodstain, saliva stain and sperm stain, as a matter of common knowledge, there are the most bloodstains. A qualitative examination is necessary before detecting DNA polymorphisms. However, it cannot •secure enough amount sample for the next DNA analysis when use up a sample in preliminary examination. Therefore in this study just examined a DNA analysis by reuse in the sample which used in preliminary examination. The PCR of 15 STR polymorphisms were amplified by a sample after the qualitative inspection of a bloodstains, saliva stains and the sperm stains, and the genotypes were able to detect from most samples.

Free Research Field

法医学