Ectopic fat accumulation and dysfunction in cultured myocytes-Regulatory role of Sirt1

2014 Fiscal Year Final Research Report

• Project/Area Number: 24590877
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General internal medicine (including Psychosomatic medicine)
• Research Institution: The University of Tokyo
• Principal Investigator: ETO Masato 東京大学, 医学部附属病院, 准教授 (80282630)
• Co-Investigator(Kenkyū-buntansha): IIJIMA Katsuya 東京大学, 高齢社会総合研究機構, 准教授 (00334384) ; OTA Hidetaka 東京大学, 医学部附属病院, 助教 (20431869)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 骨格筋 / 脂肪酸 / ビタミンD

Outline of Final Research Achievements

Sarcopenia and ectopic fat deposition is related to aging and age-associated disorders. Therefore, to develop the efficient therapeutic strategy, the molecular mechanisms need to be clarified. Aim of this project was to examine the mechanisms underlying fatty acid-induced skeletal muscle dysfunction. Treatment with high dose of fatty acids induced lipid droplet formation and apoptosis accompanied with cellular insulin resistance, in C2C12 myocytes. Vitamin D3 induced Sirt-1 upregulation and restoration of fatty acid-induced insulin resistance. Finally, isoproterenol upregulated the expression levels of VDR in C2C12 cells.

Free Research Field

内科学