2014 Fiscal Year Final Research Report
Roles for PPAR gamma in the hypothalamic PVN in the catecholamin secretion from adrenal medulla in rats
| Project/Area Number |
24590903
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General internal medicine (including Psychosomatic medicine)
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| Research Institution | Aichi Medical University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | hypothalamic PVN / preautonomic neuron / PPAR gamma / plasma catecholamine / restraint stress |
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| Outline of Final Research Achievements |
The hypothalamic paraventricular nucleus (PVN) is one of the regulatory centers for various stress responses. The present study determined whether PPARγ in the PVN is involved in the restraint stress (RS)-induced catecholamine secretion from adrenal medulla in rat. The RS-induced elevation of plasma catecholamines was suppressed by continuous intravenous infusion of 5% glucose. In contrast, pretreatment with intra peritoneal injection with GW9962, an antagonist for PPARγ, abolished the suppression effect. Western blot analyses showed the increase of levels of PPARγ protein in the hypothalamus. Further more, by immunohistochemical analyses revealed that RS gradually increased the expression of PPARγ in the dorsal part and ventral part of the PVN, where preautonomic neurons exist. These results suggest that PPARγ in the preautonomic neurons in the PVN may be involved in RS-induced elevation of plasma catecholamines in rats.
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| Free Research Field |
神経科学、特に視床下部室傍核における交感神経系の中枢制御機構
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