Validation of new risk factors for cerebral white matter lesions by proteomics

2014 Fiscal Year Final Research Report

• Project/Area Number: 24590909
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General internal medicine (including Psychosomatic medicine)
• Research Institution: National Hospital Organization, Hizen Psychiatric Center
• Principal Investigator: TAKASHIMA YUKI 独立行政法人国立病院機構肥前精神医療センター(臨床研究部), その他部局等, 研究員 (70576404)
• Co-Investigator(Kenkyū-buntansha): YAO Hiroshi 独立行政法人国立病院機構肥前精神医療センター, 臨床研究部, 生化学研究室室長 (20265010)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 大脳白質病変 / プロテオミクス / mass spectrometry / プロテインチップ

Outline of Final Research Achievements

We applied Surface-enhanced laser desorption / ionization - time-of flight mass spectrometry (SELDI -TOF-MS) technology to study proteomic profiles at serum of with white matter lesions in healthy adults. Preliminarily, we did proteo miner protocol and obtained 4 fraction of serum. Then we obtained expression of protein profiles by using strong anion-exchange (Q10) and weak cation-exchange (CM10) and Protein Chip Arrays. With the TOF-MS, mass-to-charge (m/z) or molecular weight (M.W.) of the peptide was determined. The protein expression patterns were analyzed using Biomarker Wizard software, which generates consistent peak intensities were shown as the ratio of intensity values. Result: We went five times of experiments. However, we were not able to find the significantly different peak intensity between two groups. Conclusion: There were no specific protein profiles difference in human serum with or without cerebral white matter lesions.

Free Research Field

神経内科