Basic investigation for new cytotherapy in animal model for immune mediated fulminant hepatitis.

2014 Fiscal Year Final Research Report

• Project/Area Number: 24590966
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: Nagoya University
• Principal Investigator: ISHIGAMI Masatoshi 名古屋大学, 医学部附属病院, 講師 (90378042)
• Co-Investigator(Kenkyū-buntansha): GOTO Hidemi 名古屋大学, 大学院医学系研究科, 教授 (10215501) ; ISHIKAWA Tetsuya 名古屋大学, 医学部, 教授 (10288508) ; MARUYAMA Shoichi 名古屋大学, 大学院医学系研究科, 准教授 (10362253)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 脂肪由来幹細胞 / 急性肝不全モデル / 炎症制御 / 肝再生

Outline of Final Research Achievements

We investigated the effect of LASC (low serum cultured adipose cell derived stem cells) in animal model of liver injury. The results were as below; (1) In ConA-induced acute liver injury mice, LASC showed significant suppression of serum ALT elevation compared with control mice. (2) Intrahepatic ｍRNA expression of IL-6, IL-10, IFN-γ were downregulated and TGF-βwas upregulated in LASC administrated mice compared with control mice. CD3, CD4, CD8, CD11b and CD11c mRNA expression were also downregulated. (3) In vitro culuture with mouse splenocyte, coculture with LASC downregulate the expression of CD3 in splenocyte, however, expression of NK1.1 and CD19 did not change.
These data suggested that LASC have immunomoduratory effect to supress liver injury in the animal model for acute liver injury, especially through the immunomodulatory effect for T cells.

Free Research Field

肝臓病学