2014 Fiscal Year Final Research Report
The analysis of small bone marrow drived liver repair cell
| Project/Area Number |
24590978
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Yamaguchi University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
TERAI Shuji 新潟大学, 医歯学系研究科, 教授 (00332809)
TAKAMI Taro 山口大学, 大学院医学系研究科, 講師 (60511251)
FUJISAWA Koichi 山口大学, 大学院医学系研究科, 助教 (00448284)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | 再生医療 / 電子顕微鏡 / 肝線維化 / 骨髄細胞 / 幹細胞 / 免疫電顕 / CCL4モデル |
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| Outline of Final Research Achievements |
We developed the GFP/CCl4 model which monitor the GFP-positive bone marrow cell (BMC) repopulated under CCl4 induced liver cirrhosis mice.We found two kinds of GFP positive BMCs in recipient cirrhosis liver using IEM method. One group of GFP positive BMCs was similar to hepatocyte in size(15-30um) and located around fiber（MMP9 positive cell）.The other group cells were small size (2-5microum) and located in destructive area（A6 positive cell, Liv2 positive cell）. And EpCAM positive cells were included. These cells were circular forms and had high N/C ratio and smaller than hepatocyte. We separated EpCAM-positive and EpCAM-negative cells and then transplanted these cells into a continuous liver damaged model（CCl4 model). At 4 weeks after BMC transplantation, the ratio of liver fibrosis in EpCAM-negative cells injected liver was decreased better than EpCAM-positive cells.
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| Free Research Field |
消化器内科学
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