2014 Fiscal Year Final Research Report
Role of HCV and periostin on the regulation of liver cancer stem cells
| Project/Area Number |
24590994
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Keio University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | 癌幹細胞 / HCV / Periostin |
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| Outline of Final Research Achievements |
The aims of the study are to investigate the role of HCV virus on the liver cancer stem cell (CSC) biology; and to investigate the role of periostin on the liver carcinogenesis. The novel findings of the manuscript include: 1) CD133+/SP subpopulation is a distinct CSC population in Huh7 cells; 2) transduction of HCV full-length replicon or HCV core protein expands CSC population and augments CSC properties through two different mechanisms including (a) HCV induced expansion of CSC population through potentiation of self-renewal capacity and (b) transition from non-CSC CD133-/SP cells to CSC CD133+/SP cells; 3) HCV-mediated expansion of CSC population and augmentation of CSC properties are mediated by HIF-1α stabilization; 4) periostin KO mice did not develop CCL4-induced liver fibrosis as compared with WT mice; and 5) periostin KO mice did not develop DEN-induced liver tumors.
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| Free Research Field |
消化器内科
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