2014 Fiscal Year Final Research Report
Investigation of primary culture system for pancreatic cancer stem cells
| Project/Area Number |
24591017
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Keio University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
SATO Toshiro 慶應義塾大学, 医学部, 特任准教授 (70365245)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | 癌幹細胞 / 上皮間葉転換 / CD44 / 膵癌 / 間葉系幹細胞 |
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| Outline of Final Research Achievements |
Primary culturing or long-term culturing of pancreatic cancer tissue is important technology for the study of cancer biology and investigation of molecular specific treatment strategy. We performed long-term culturing of primary pancreatic cancer tissue using matrigel 3D culture system. The pancreatic cancer cells were enriched with CD44-sprice variant-positive cells, so-called cancer stem cell-like population. In addition, we found that co-culturing of pancreatic cancer cells with mesenchymal stem cells (MSCs) enhanced cancer stem cell-like properties as well as epithelial-to-mesenchymal transition within the cancer cells. The matrigel 3D culture system or co-culturing with MSCs is an attractive technology for in vitro analysis of cancer biology.
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| Free Research Field |
腫瘍学
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