Generation of artificial pancreatic cancer stem cell and its regulation by microRNA in order to develop effective therapy for pancreatic cancer.

2014 Fiscal Year Final Research Report

• Project/Area Number: 24591022
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: Miyagi Prefectural Hospital Organization Miyagi Cancer Center
• Principal Investigator: KENNICHI Satoh 地方独立行政法人宮城県立病院機構宮城県立がんセンター(研究所), がん幹細胞研究部, 部長 (10282055)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 膵癌 / 人工がん幹細胞 / マイクロＲＮＡ

Outline of Final Research Achievements

In order to develop effective therapy for pancreatic cancer, we tried to generate artificial pancreatic cancer stem cell. Human pancreatic duct cell line (HPDE) transfected with expression vector of activated K-ras demonstrated the increased sphere formation but could not show the increase in number of cancer stem cell marker positive cells. PKM2, a key molecule for Warburg effect, was significantly expressed in pancreatic cancer cells compared to normal cells. In addition, suppression of PKM2 expression resulted in decreased cellular growth. During the exploration for microRNA associated with cancer stem cell property, miR-483-3p was identified as highly expressed microRNA in pancreatic cancer cells.

Free Research Field

消化器内科学