2014 Fiscal Year Final Research Report
The role of midkine in the onset and progression of aortic stenosis
| Project/Area Number |
24591045
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Circulatory organs internal medicine
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| Research Institution | Hamamatsu University School of Medicine |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
SATOH Hiroshi 浜松医科大学, 医学部附属病院, 講師 (30293632)
SAOTOME Masao 浜松医科大学, 医学部附属病院, 助教 (70509512)
KATOH Hideki 浜松医科大学, 医学部, 助教 (80314029)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | 大動脈狭窄症 / midkine / 免疫染色 / mRNA / 石灰化 |
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| Outline of Final Research Achievements |
The purpose of this study was to investigate the role of midkine in the progression of aortic stenosis (AS). From the preparation after operation of AS, the existence of midkine was confirmed in valve interstitial cell (VIC) by HE-stain and immnostaining of midkine. Next, in many preparations of aortic valve from autopsied patients showed the existence of midkine. In many patients who showed the staining of midkine without AS had malignancy or severe infectious disease.
We, next, investigated the expression of mRNA of midkine using RT-PCR method, but the density of VIC in the valve was too low to identify the mRNA of midkine, because of severe calcification of the valve.
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| Free Research Field |
循環器内科
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