Development of a PTX3 absorption column for patients with SIRS.

2017 Fiscal Year Final Research Report

• Project/Area Number: 24591070
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Circulatory organs internal medicine
• Research Institution: Juntendo University
• Principal Investigator: INOUE KENJI 順天堂大学, 医学部, 准教授 (80317388)
• Project Period (FY): 2012-04-01 – 2018-03-31
• Keywords: 敗血症 / 全身性炎症反応症候群 / 体外循環式カラム

Outline of Final Research Achievements

To overcome a non-specific absorption with histone to pentraxin 3 (PTX3) columns, we have studied which sequence of PTX3 was most appropriate or 2) which beads or blocking buffer were appropriate to reduce the background. However, we could not find an appropriate condition to reduce it. But an electro-microscopy revealed a very strong aggregation of recombinant PTX3 and histone 4, which may cause the non-specific absorption. From this finding, we consider that the mechanism of a strong aggregation is the fact that PTX3 inhibit histone, which cause to damage of human endothelial cell in septic condition.

Free Research Field

循環器内科学