New diagnostic and therapeutic strategies for atherosclerosis using newly developed apolipoprotein A-I mimetic peptide

2014 Fiscal Year Final Research Report

• Project/Area Number: 24591123
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Circulatory organs internal medicine
• Research Institution: Fukuoka University
• Principal Investigator: SAKU Keijiro 福岡大学, 医学部, 教授 (40183371)
• Co-Investigator(Kenkyū-buntansha): OHTA Takao 琉球大学, 医学部, 教授 (70185271) ; MIURA Shinichiro 福岡大学, 医学部, 准教授 (20343709)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: ApoA-I模倣ペプチド / HDL / コレステロール逆転送系

Outline of Final Research Achievements

We had developed apolipoprotein A-I mimetic peptide (Fukuoka University ApoA-I Mimetic Peptide, FAMP).In this project,we established new diagnostic and therapeutic strategies for atherosclerosis using FAMP.First,FAMP had an anti-atherosclerotic effect through the enhancement of function of HDL in mice model of atherosclerosis.Second,as the mechanisms of FAMP-indiced atheroprotective effects,we found that the administration of FAMP promoted ATP-binding cassette transporter A1-dependent efflux ex vivo,HDL turnover in vivo, and macrophage reverse cholesterol transport in vivo despite reduced plasma HDL-C levels.In addition, HDL with FAMP evoked pleiotropic effects,such as improvement of cardiac function in a model of myocardial ischemia reperfusion injury, an induction of endothelial cell tube formation and anti-inflammation. Finally, using FAMP as a diagnostic tool, FAMP with 68Ga-DOTA is a promising candidate diagnostic tracer for PET imaging of the atherosclerotic lipid burden.

Free Research Field

医歯薬学