Type 1-polarized dendritic cells pulsed with ESAT-6 are a potent immunogen against tuberculosis

2014 Fiscal Year Final Research Report

• Project/Area Number: 24591160
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Respiratory organ internal medicine
• Research Institution: Hamamatsu University School of Medicine
• Principal Investigator: YUTARO Nakamura 浜松医科大学, 医学部附属病院, 講師 (60436962)
• Co-Investigator(Kenkyū-buntansha): SUDA Takafumi 浜松医科大学, 医学部, 教授 (30291397) ; NAGATA Toshi 浜松医科大学, 医学部, 教授 (90275024)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 結核 / 樹状細胞 / 細胞性免疫 / ワクチン

Outline of Final Research Achievements

The development of effective vaccine strategies for tuberculosis(TB) is one of the major frontiers of medical research. Our previous studies showed that type-1 polarized dendritic cell (alphaDC1) vaccine is a promising approach for eliciting protective immunity against intracellular bacteria in animal model. Here we showed that alpha DC1 from TB patients could significantly induce antigen specific type-1 immunity. Monocyte-derived DCs from TB patients were induced to mature using a "standard" cytokine cocktail or using an alpha-type 1-polarized DC (alphaDC1) cocktail and were pulsed with established TB antigen (ESAT-6). AlphaDC1 secreted substantially higher levels of IL-12p70 than standard DCs. Furthermore alphaDC1 induced much higher numbers of interferon gamma producing functional T cells against TB. The current demonstration that TB antigen pulsed alphaDC1 are potent inducers of TB-specific T cells helps to develop improved immunotherapies.

Free Research Field

呼吸器内科学