The significance of signaling pathways by exososme in lung injury and fibrosis

2014 Fiscal Year Final Research Report

• Project/Area Number: 24591178
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Respiratory organ internal medicine
• Research Institution: Jikei University School of Medicine
• Principal Investigator: KUWANO Kazuyoshi 東京慈恵会医科大学, 医学部, 教授 (40205266)
• Co-Investigator(Kenkyū-buntansha): NAKAYAMA Katsutoshi 東京慈恵会医科大学, 呼吸器内科, 教授 (40321989) ; ARAYA Jun 東京慈恵会医科大学, 呼吸器内科, 教授 (90468679)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: ＣＯＰＤ / ｐｕｌｍｏｎａｒｙ ｆｉｂｒｏｓｉｓ / ｓｅｎｅｓｃｅｎｃｅ / ｍｉｃｒｏ ＲＮＡ / ｅｘｏｓｏｍｅ / ｌｕｎｇ ｉｎｊｕｒｙ

Outline of Final Research Achievements

AIM: To verify the role of microRNA in COPD and IPF pathogenesis, especially the regulation of cellular senescence and SASP using exososme
1. Extraction and analysis of exosome in BALF from patients with COPD and IPF：Exosome was isolated from BALF using ultracentrifuge anda filtration. Exosome obtained from BALF was successfuly obtained and suitable for analysis.
2. Analysis of microRNA using bronchial epithelial cells and fibroblasts：　isolated from patients with lung cancetr.　microRNA isolated by microarray analysis were associated with senescence induction in bronchial epithelial cells.

Free Research Field

呼吸器内科学