Directed differentiation of collecting duct cells from human iPS cells towards the creation of novel in vitro models for cystic kidney diseases

2014 Fiscal Year Final Research Report

• Project/Area Number: 24591195
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Kidney internal medicine
• Research Institution: Kyoto University
• Principal Investigator: OSAFUNE Kenji 京都大学, iPS細胞研究所, 教授 (80502947)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 腎臓 / 尿管芽 / 中間中胚葉 / 集合管 / iPS細胞 / ES細胞 / ADPKD / ARPKD

Outline of Final Research Achievements

The directed differentiation methods from human ES or iPS cells into renal lineage cells have not yet been established. In this study, we aimed to develop a differentiation protocol from human iPS cells into collecting duct cells in order to create disease models for autosomal dominant polycystic kidney disease (ADPKD) and autosomal recessive polycystic kidney disease (ARPKD). By using combinatorial treatments of growth factors, we partially succeeded in generating collecting duct cells from human iPS cells through intermediate mesoderm and ureteric bud. We will aim to establish an efficient differentiation method to generate collecting duct cells from human iPS cells and create novel in vitro disease models for ADPKD and ARPKD by differentiating the patient-derived iPS cells into collecting duct cells.

Free Research Field

腎臓再生