2014 Fiscal Year Final Research Report
Development of PI polyamide targeting FcRg for nephritis
| Project/Area Number |
24591216
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Kidney internal medicine
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| Research Institution | Nihon University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
FUKUDA Noboru 日本大学, 総合科学研究科, 教授 (40267050)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | Fc受容体 / ピロール・イミダゾールポリアミド / 全身性エリテマトーデス / 腎炎 / 遺伝子治療 |
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| Outline of Final Research Achievements |
FcRγ chain (Fecr1g) is a common intra cellular subunit of Fc receptors, which is involved in the autoimmuno diseases. We synthesized a novel gene silencer PI polyamide targeting FcRγ chain for the immunological nephritis. In in vitro experiments, the PI polyamide targeting FcRγ chain inhibited expression of FcRγ chain mRNA and FcRγ chain promoter activity in cultured J774A.1 cells. In in vivo experiments, iv administered PI polyamide targeting FcRγ chain suppressed CD16/32 positive cells. Moreover, Syc inhibitor R406 suppressed glomerulonephritis in the anti-GBM antibody-induced nephritis and lupus nephropathy in SLE model mouse. We will further develop the PI polyamides targeting FcRγ chain as potential therapeutic agents for immunological nephritis.
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| Free Research Field |
腎臓内科
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