Searching for autoantibodies related Alzheimer disease by proteomics and protein array system

2014 Fiscal Year Final Research Report

• Project/Area Number: 24591256
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Neurology
• Research Institution: Gifu University
• Principal Investigator: INUZUKA Takashi 岐阜大学, 医学(系)研究科(研究院), 教授 (50184734)
• Co-Investigator(Kenkyū-buntansha): KIMURA Akio 岐阜大学, 大学院医学系研究科, 准教授 (00362161) ; YANO Takeo 三重大学, 地域イノベーション学研究科, 教授 (00569197)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: アルツハイマー型認知症 / 抗血管内皮抗体 / 抗神経抗体 / バイオマーカー / プロテオミクス

Outline of Final Research Achievements

We identified novel autoantibodies in serum samples from patients with Alzheimer’s diseases. To identify these novel antibodies, we employed two-dimensional immunoblotting using human neuroblastoma cells, human brain microvascular endothelial cells, or rat brain homogenate as the antigen source. As a result, we identified autoantibodies against Phosphatidylinositol transfer protein alpha isoform and Mitochondrial import receptor subunit TOM40 homolog in sera from patients with Alzheimer’s diseases. There are several reports describing that these autoantigens may be associated with Alzheimer’s diseases. Further studies are needed to establish whether our identified autoantibodies could be useful biomarkers of Alzheimer’s diseases.

Free Research Field

内科系臨床医学・神経内科学