Functional analysis of G-protein coupled receptor 3 for future applications of novel stroke therapy utilizing neuronal stem cell transplantation

2014 Fiscal Year Final Research Report

• Project/Area Number: 24591264
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Neurology
• Research Institution: Hiroshima University
• Principal Investigator: TANAKA Shigeru 広島大学, 医歯薬保健学研究院, 講師 (20512651)
• Co-Investigator(Kenkyū-buntansha): MATSUMOTO Masayasu 広島大学, 医歯薬保健学研究院, 教授 (20192346)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: GPCR / cAMP / PKA

Outline of Final Research Achievements

G-protein coupled receptor 3 (GPR3) belongs to a member of constitutively active Gs-coupled receptors that activate 3’-5’-cyclic adenosine monophosphate (cAMP) and is highly expressed in the brain. In the current study, principal investigator has clarified that GPR3 was distributed in the cortex, striatum, hippocampus, medial habenular nucleus, and cerebellum. The GPR3 subcellular localization analysis using fluorescent-tagged GPR3 revealed that GPR3 was distributed along the plasma membrane and in goldi bodies and endosomes in neurons. Moreover, time-lapse experiments revealed that fluorescent punctae of GPR3 were transported along the neurite, which was associated with the local activation of PKA at the neurite tips.

Free Research Field

医歯薬学