Analysis for new target gene of Mafa, and application for pancreatic beta-cell generation.

2014 Fiscal Year Final Research Report

• Project/Area Number: 24591328
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Metabolomics
• Research Institution: Osaka University
• Principal Investigator: MATSUOKA Takaaki 大阪大学, 医学(系)研究科(研究院), 講師 (10379258)
• Co-Investigator(Kenkyū-buntansha): MIYATSUKA Takeshi 大阪大学, 医学系研究科, 特任講師 (60622363) ; KANETO Hideaki 川崎医科大学, 医学部, 教授 (80448034)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 糖尿病 / ｲﾝｽﾘﾝ合成 / ｲﾝｽﾘﾝ転写因子

Outline of Final Research Achievements

it is still unknown how Mafa is involved in insulin secrestion. To examine its mechanism, we performed ChIP assay with Mafa antibody and MIN6 cells followed by deep sequencing of isolated fragmented DNA. As a result, we could isolate several genes as target of Mafa. At least, one of them is clearly important for generating ATP and glucose-induced insulin secretion in pancreatic -cells. In another study, To explore the potential role of Mafa in reprogramming capacity in vivo, we generated transgenic mice conditionally expressing insulin transcription factors by the Cre/loxP system. Ectopic and combined expressions of those factors induced insulin producing cells from various non-beta-cells in vivo. Interestingly, the difference of original cell make different efficiency for the reprograming to insulin producing cells. These results demonstrated the critical role of Mafa for beta-cell function and generation.

Free Research Field

糖尿病