Novel immunosuppressive therapy of type 1 diabetes mellitus using adipocyte-derived dedifferentiated fat (DFAT) cells

2014 Fiscal Year Final Research Report

• Project/Area Number: 24591344
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Metabolomics
• Research Institution: Nihon University
• Principal Investigator: ISHIGE Mika 日本大学, 医学部, 助教 (90420950)
• Co-Investigator(Kenkyū-buntansha): MATSUMOTO Taro 日本大学, 医学部, 教授 (50366580) ; MUGISHIMA Hideo 日本大学, 医学部, 教授 (80183648) ; URAKAMI Tatsuhiko 日本大学, 医学部, 准教授 (60223616)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 再生医療 / 1型糖尿病 / 免疫抑制療法

Outline of Final Research Achievements

In this study, we examined efficient induction of mice adipocyte-derived dedifferentiated fat (DFAT) cells, which were prepared from mature adipose tissue of nonobese diabetic (NOD) mice with autoimmune type 1 diabetes. The results showed that DFAT cells prepared form NOD (NOD-DFAT) expressed same surface markers as Mesenchymal stem cells (MSC). Mice treated with NOD-DFAT cells increased their lifespan, as compared with untreated mice. MSC from human umbilical cords showed dose-dependent effect of inhibition of T cell proliferation and mRNA expression of several immunosuppressive factors. These results suggest that DFAT cells may be an attractive new immunosuppressive therapy source for cell-based therapy in young patients with type 1 diabetes.

Free Research Field

再生医療