The humatopoietic stem cell induction from human embryonic stem cells

2014 Fiscal Year Final Research Report

• Project/Area Number: 24591381
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Hematology
• Research Institution: Kyoto University (2013-2014); Chiba University (2012)
• Principal Investigator: OSAWA Mitsujiro 京都大学, iPS細胞研究所, 特定助教 (70546770)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 造血幹細胞

Outline of Final Research Achievements

To understand the molecular basis of hematopoietic differentiation from human pluripotent stem cell included human embryonic stem cells and human induced pluripotent stem cells, we first analyzed a hematopoietic phenotype and gene expression on human ES-derived hematopoietic progenitor cells. As a result, hematopoietic progenitor cells with long-term culture showed developmental maturation of hematopoietic. We also evaluated the effects of overexpression of key molecules predicted to support the generation and maturation of HSCs. Among genes tested, we found that only SOX17 plays a critical role in priming hemogenic potential of endothelial cells.

Free Research Field

幹細胞生物学